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National Repository of Grey Literature 4 records found  Search took 0.00 seconds. 
Transfection of somatic cells using magnetic nanoparticles and their post-transfection localization
 Pošustová, Veronika ; Skopalík, Josef (referee) ; Svoboda, Ondřej (advisor)
This bachelor thesis is focused on an experiment based on magnetic particles SPIO for transfection of 3T3 cells. Nanoparticle surface was modified with PEI and Chitosan to optimize this method. The transfection experiment was performed twelve times, then the scanned data was processed and statistically analyzed in Matlab R2016a to determine the transfection efficiency. The theoretical part is focused on magnetic nanoparticles and their application in medicine, on transfection and in last part on a transfection of cells using magnetic nanoparticles.
Detailed record
Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells
 Bílek, Ondřej ; Svoboda, Ondřej (referee) ; Fohlerová, Zdenka (advisor)
This bachelor’s thesis deals with using of magnetic nanoparticles, SPIO nanoparticles and nanoparticles of commercial product MATra for transfection of ASAP plasmid into HEK293 cells and subsequent optimization of the process. The experimental part is enclosed with theorethical part that summarizes findings from the fields of synthesis of magnetic nanoparticles and their biomedical applications, theory dealing with possibilities of the insertion of foreign DNA or plasmids into the cell and of its efficiency evaluation.
Detailed record
Transfection of somatic cells using magnetic nanoparticles and their post-transfection localization
 Pošustová, Veronika ; Skopalík, Josef (referee) ; Svoboda, Ondřej (advisor)
This bachelor thesis is focused on an experiment based on magnetic particles SPIO for transfection of 3T3 cells. Nanoparticle surface was modified with PEI and Chitosan to optimize this method. The transfection experiment was performed twelve times, then the scanned data was processed and statistically analyzed in Matlab R2016a to determine the transfection efficiency. The theoretical part is focused on magnetic nanoparticles and their application in medicine, on transfection and in last part on a transfection of cells using magnetic nanoparticles.
Detailed record
Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells
 Bílek, Ondřej ; Svoboda, Ondřej (referee) ; Fohlerová, Zdenka (advisor)
This bachelor’s thesis deals with using of magnetic nanoparticles, SPIO nanoparticles and nanoparticles of commercial product MATra for transfection of ASAP plasmid into HEK293 cells and subsequent optimization of the process. The experimental part is enclosed with theorethical part that summarizes findings from the fields of synthesis of magnetic nanoparticles and their biomedical applications, theory dealing with possibilities of the insertion of foreign DNA or plasmids into the cell and of its efficiency evaluation.
Detailed record

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